南京医科大学学报（英文版）2004，Vol.18Issue(6)：323-325,3.
雷帕霉素对异种单向混合淋巴细胞培养的免疫抑制作用
Effect of New Immunosuppressant-Rapamycin on One-way Mixed Lymphocyte Culture
夏国伟 1张元芳 2丁强2
作者信息
- 1. 南京医科大学第一附属医院泌尿科,南京,210029,中国
- 2. 复旦大学附属华山医院泌尿科,上海,200040,中国
- 折叠
摘要
Abstract
Objective: Observing human to mouse one-way mixed lymphocyte culture(xMIC) and the effect of new immunosuppressant-Rapamycin on XMIC. Methods: Mouse splenic lymphocyte were collected and treated by mitomycin as activating cell; Human Peripheral blood lymphocytes(hPBL)were separated and gathered as reacting cell;Mouse splenic lymphocyte and hPBL wee mixed to incubate for 1 week; The researchers designed control 、RPM groups,and experiment(drugs) grup have different concentration. Results: HPBL in the experiment groups (mixed mouse lymphocyte)proliferated obviously, the amount of3 H-TdR in corporation increased evidently( P ＜ 0.05, The mean percentage of CD4, CD8, LgG, LgM positive cells rose markedly. HPBL in the experiment groups less proliferated, the amount of 3H-TdR incorporation declined,RPM' s ic50 (50% inhibition concentration)approximately in 1.5 nmol/L; the mean percentage of CD4, CD8, IgG, IgM positive cells fell obviously. Conclusion: The human to mouse one-way MLC has obvious lymphocyte proliferation. New immunosuppressants-Rapamycin have powerful effete on XMLC.关键词
异种混合淋巴细胞培养/免疫抑制剂/雷帕霉素Key words
Mixed lymphocyte culture/immunosuppressant/rapamycin分类
医药卫生引用本文复制引用
夏国伟,张元芳,丁强..雷帕霉素对异种单向混合淋巴细胞培养的免疫抑制作用[J].南京医科大学学报（英文版）,2004,18(6):323-325,3.
