首页|期刊导航|中国农业科学（英文版）|Somatic Embryogenesis and Plant Regeneration from Two Recalcitrant Genotypes of Gossypium hirsutum L.
中国农业科学（英文版）2006，Vol.5Issue(5)：323-329,7.
Somatic Embryogenesis and Plant Regeneration from Two Recalcitrant Genotypes of Gossypium hirsutum L.
Somatic Embryogenesis and Plant Regeneration from Two Recalcitrant Genotypes of Gossypium hirsutum L.
摘要
Abstract
An improved protocol has been developed for somatic embryogenesis and plant regeneration of recalcitrant cotton cultivars. High callus frequencies and embryogenic tissue were developed in MSB medium supplemented with gradient concentrations of KT and 2,4-D, their concentration decreasing from 0.1 to 0.01 mg L-1. Somatic embryos were successfully incubated in 1/2 macronutrient MSB suspension supplemented with 0.5 g L-1 glutamine and 0.5 g L-1 asparagine. Decrease in macronutrient concentration of MSB significantly alleviated browning and was beneficial to suspension cells.Transformation of somatic embryos into plants was induced in MSB medium supplemented with 3% sucrose, 0.5 g L-1 glutamine, 0.5 g L-1 asparagine, and 6.0 g L-1 agar. The effect of sucrose as carbohydrate was better than that of glucose for plant germination. Using this protocol, regenerated plantlets from the CCRI521 and Zhongzhi86-6 reached to as much as 19.6 and 18.5% somatic embryos, respectively.关键词
Gossypium hirsutum L./recalcitrant genotypes/plant regeneration/somatic embryogenesis/effect of macronutrients/suspension cultureKey words
Gossypium hirsutum L./recalcitrant genotypes/plant regeneration/somatic embryogenesis/effect of macronutrients/suspension culture分类
农业科技引用本文复制引用
WANG Yan-xia,WANG Xing-fen,MA Zhi-ying,ZHANG Gui-yin,HAN Gai-ying..Somatic Embryogenesis and Plant Regeneration from Two Recalcitrant Genotypes of Gossypium hirsutum L.[J].中国农业科学（英文版）,2006,5(5):323-329,7.基金项目
This research was supported by grants from the China National Basic Research Program (2004CB 117302-1). （2004CB 117302-1）
