草业科学2009，Vol.26Issue(7)：32-35,4.
诱变处理苜蓿愈伤组织抗碱性的研究
Study on alkalinity resistance of alfalfa callus in mutagenic treatments
摘要
Abstract
The stem of alfalfa(Medicago sativa)was used as the experimental material, the inducement of callus was conducted, and the callus of alfafa was treated by ultraviolet ray and NaN3. Callus in mutagenic treat and control were filtrated by proline and forced alkalinity stress by NaHCO3 and Na2CO3, then they were appraised by the alkalinity resistance physiological indexes such as peroxidase activity, soluble protein content and proline content. It was shown that all these three indexes in the treated callus were evidently higher than those in the control. So the method of ultraviolet ray and NaN3 could be used to improve the alkalinity resistance of alfalfa, and ultraviolet ray treatment had greater effect on the alkali resistance of alfalfa than NaN3 treatment.关键词
NaN3/苜蓿/愈伤组织/抗碱性Key words
NaN3/ alfalfa/ callus/ alkali resistance分类
农业科技引用本文复制引用
李红,李波,赵洪波,王影..诱变处理苜蓿愈伤组织抗碱性的研究[J].草业科学,2009,26(7):32-35,4.基金项目
国家科技支撑计划子课题"优质高产抗逆苜蓿新品种选育"(2006BAD04A04-01) （2006BAD04A04-01）
"十一五"黑龙江省科技厅重点农业攻关项目"优质牧草新品种选育及生产配套技术研究"(GB06B109) （GB06B109）
