福建林学院学报2001,Vol.21Issue(1):32-35,4.
锥栗种子离体胚超低温保存脱氢酶活性研究
Study on Dehydrogenase Activity of Excised Embryos of
Castanea Henryi Seeds after Cryopreservation
摘要
Abstract
By applying cryopreservation(-196 ℃)treatment and using the methods of variance analysis and multiple comparison of Q test of TTCH content of excised embryos after cryopreservation, dehydrogenase activity of excised embryos was analysed and long-term storage feasibility was studied. The results showed moisture content(MC) was the main factors affecting the cryopreservation of C. henryi excised embryos, and the measures desiccating a little down to a medium MC should be taken in cryopreservation. Under the conditions of no cryoprotectants pretreatment, 20% MC, mild freezing, and quick thawing, the dehydrogenase activity of excised embryos was the highest.关键词
锥栗/种子贮藏/离体胚/超低温保存/脱氢酶活性分类
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陈礼光,郑郁善..锥栗种子离体胚超低温保存脱氢酶活性研究[J].福建林学院学报,2001,21(1):32-35,4.