南京医科大学学报（英文版）2006，Vol.20Issue(6)：325-330,6.
Cytotoxicity of acrylamide and its epoxide glycidamide in CHO cells expressing human cytochrome P450 2E1
Cytotoxicity of acrylamide and its epoxide glycidamide in CHO cells expressing human cytochrome P450 2E1
Shoulin Wang 1Xiaoyang He 2Xinru Wang 1Junyan Hong2
作者信息
- 1. Institute of Toxicology, Nanjing Medical University, Nanjing 210029, China
- 2. School of Public Health, University of Medicine and Dentistry of New Jersey, Piscataway, NJ 08854, USA
- 折叠
摘要
Abstract
Objective: To investigate whether CYP2E1 is responsible for the acrylamide metabolic activation in Flp-In CHO cell system. Methods: CYP2E1 cDNA was subcloned from the human liver full-length cDNA library and subsequently transfected into the Flp-In CHO cells to generate the stable transfectant of CYP2E1. The CYP2E1 mRNA expression was determined by RT-PCR. Acrylamide and its epoxide glycidamide induced cytotoxicity and cell cycle arrest in G2/M were conducted using MTS assay and flow cytometry, respectively. Results: In the CHO cell stably expressing CYP2E1 (CHO-2E1), a ～1.5 kbsize of band was detected from the mRNA in the cells while no corresponding band in the CHO-vector cells, which indicated that CYP2E1 was successfully transfected in the CHO cells. Compared with the CHO-vector cells, acrylamide showed a concentrationdependent loss of viability in the CHO-2E1 cells but no significant change of G2/M arrest was found. As expected, glycidamide induced similar profile of cytotoxicity in both of the cells, and G2/M arrest presented a concentration-dependent increased in the CHO-2E1 cells. Conclusion: The result suggested that CYP2E1 might be responsible for the acrylamide metabolism, and its metabolite glycidamide was a direct cytotoxic and genotoxic agent. It should be further considered whether acrylamide-induced toxicity is through its epoxide glycidamide in the presence of CYP2E1.关键词
Flp-In CHO cells/cytochrome P450 2E1/cytotoxicity/acrylamide/glycidamideKey words
Flp-In CHO cells/cytochrome P450 2E1/cytotoxicity/acrylamide/glycidamide分类
医药卫生引用本文复制引用
Shoulin Wang,Xiaoyang He,Xinru Wang,Junyan Hong..Cytotoxicity of acrylamide and its epoxide glycidamide in CHO cells expressing human cytochrome P450 2E1[J].南京医科大学学报（英文版）,2006,20(6):325-330,6.
