神经损伤与功能重建2008，Vol.3Issue(5)：328-341,14.
Morphogenesis and Regulation of Bergmann Glial Processes During Purkinje Cell Dendritic Spine Ensheathment and Synaptogenesis
Morphogenesis and Regulation of Bergmann Glial Processes During Purkinje Cell Dendritic Spine Ensheathment and Synaptogenesis
摘要
Abstract
Astrocytes have an important role in synaptic formation and function but how astrocytic processes be-come associated with synaptic structures during development is not well understood. Here we analyzed the pattern of growth of the processes extending off the main Bergmann glial (BG) shafts during synaptogenesis in the cerebellum.We found that during this period, BG process outgrowth was correlated with increased ensheathment of dendritic spines. Inaddition, two-photon time-lapse imaging revealed that BG processes were highly dynamic, and processes became more stable as the period of spine ensheathment progressed. While process motility was dependent on actin polymerization, activity of cytoskeletal regulators Racl and RhoG did not play a role in glial process dynamics or density, but was critical for maintaining process length. We extended this finding to probe the relationship between process morphology and ensheathment, finding that shortened processes result in decreased coverage of the spine.Furthermore, we found that areas in which BG expressed dn-Racl, and therefore had a lower level of synaptic en-sheathment, showed an overall increase in synapse number. These analyses reveal how BG processes grow to sur-round synaptic structures, elucidate the importance of BG process structure for proper development of synaptic en-sheathment, and reveal a role for ensheathment in synapse formation. V 2008 Wiley-Liss, Inc.关键词
Bergmann胶质细胞/树突棘/突触发生/Purkinje细胞/小脑/RaclKey words
Bergmann glia/dendritic spines/synaptogenesis/Purkinje cell/cerebellum/Racl分类
医药卫生引用本文复制引用
JOCELYN J. LIPPMAN,TAMAR LORDKIPANIDZE,MARGARET E. BUELL,SUNG OK YOON,ANNA DUNAEVSKY..Morphogenesis and Regulation of Bergmann Glial Processes During Purkinje Cell Dendritic Spine Ensheathment and Synaptogenesis [J].神经损伤与功能重建,2008,3(5):328-341,14.基金项目
Carol Mason and Kimberly Harms for helpful comments on the manuscript （）
Chavvy Tep for the Racl activity assays （）
Whitehall Foundation and NINDS(1R01NS057667) （1R01NS057667）
