神经损伤与功能重建2009，Vol.4Issue(1)：33-47,15.
NG2 Cell Response in the CNP-EGFP Mouse After Contusive Spinal Cord Injury
NG2 Cell Response in the CNP-EGFP Mouse After Contusive Spinal Cord Injury
摘要
Abstract
NG2+ cells in the adult CNS are a heterogeneous population. The extent to which the subpopulation of NG2+ cells that function as oligodendrocyte progenitor cells (OPCs) respond to spinal cord injury (SCI) and reca-pitulate their normal developmental progression remains unclear. We used the CNP-EGFP mouse, in which oligo-dendrocyte lineage cells express EGFP, to study NG2+ cells in the normal and injured spinal cord. In white matter of uninjured mice, bipolar EGFP+ NG2+ cells and multipolar EGFPnegNG2+ cells were identified. After SCI, EG-FP+ NG2+ cell proliferation in residual white matter peaked at 3 days post injury (DPI) rostral to the epicenter, while EGFPnegNG2+ cell proliferation peaked at 7 DPI at the epicenter. The expression of transcription factors, Olig2, Sox10, and Sox17, and the basic electrophysiological membrane parameters and potassium current phenotype of the EGFP+ NG2+ population after injury were consistent with those of proliferative OPCs during development. EGFPnegNG2+ cells did not express transcription factors involved in oligodendrogenesis. EGFP+ CC1+ oligodendro-cytes at 6 weeks included cells that incorporated BrdU during the peak of EGFP+ NG2+ cell proliferation. EGFPneg CC1 + oligodendroeytes were never observed. Treatment with glial growth factor 2 and fibroblast growth factor 2 en-hanced oligodendrogenesis and increased the number of EGFneg NG2+ cells. Therefore, based on EGFP and tran-scription factor expression, spatiotemporal proliferation patterns, and response to growth factors, two populations of NG2+ cells can be identified that react to SCI. The EGFP+ NG2+ cells undergo cellular and physiological changes in response to SCI that are similar to those that occur in early postnatal NG2+ cells during developmental oligoden-drogenesis. C 2008 Wiley-Liss, Inc.关键词
少突胶质前体细胞/olig2/CNP基因/内源性修复/细胞增殖/神经胶质生长因子/成纤维细胞生长因子Key words
oligodendrocyte progenitor cells/olig2/CNP gene/endogenous repair/cell proliferation/glial growth factor/fibroblast growth factor分类
医药卫生引用本文复制引用
JUDITH M LYTLE,RAMESH CHITTAJALLU,JEAN R WRATHALL,AND VITTORIO GALLO..NG2 Cell Response in the CNP-EGFP Mouse After Contusive Spinal Cord Injury[J].神经损伤与功能重建,2009,4(1):33-47,15.基金项目
We are particularly thankful to Drs.Joshua Corbin and Tarik Haydar for discussion and for critical comments on this project.We thank Drs.Thomas Finn and Gerard Ahern for discussion.We also thank all members of the Gallo and Wrathall labs for their support and discussion.Lastly,we thank Acorda for providing the rhGGF2. （）
