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Preliminary study on mitochondrial 16S rRNA gene sequences and phylogeny of flatfishes (Pleuronectiformes)

中国海洋湖沼学报(英文版)2005,Vol.23Issue(3):335-339,5.
中国海洋湖沼学报(英文版)2005,Vol.23Issue(3):335-339,5.

Preliminary study on mitochondrial 16S rRNA gene sequences and phylogeny of flatfishes (Pleuronectiformes)

Preliminary study on mitochondrial 16S rRNA gene sequences and phylogeny of flatfishes (Pleuronectiformes)

1

作者信息

  • 1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
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摘要

Abstract

A 605 bp section of mitochondrial 16S rRNA gene from Paralichthys olivaceus, Pseudorhombus cinnamomeus, Psetta maxima and Kareius bicoloratus, which represent 3 families of Order Pleuronectiformes was amplified by PCR and sequenced to show the molecular systematics of Pleuronectiformes for comparison with related gene sequences of other 6 flatfish downloaded from GenBank. Phylogenetic analysis based on genetic distance from related gene sequences of 10 flatfish showed that this method was ideal to explore the relationship between species, genera and families. Phylogenetic trees set-up is based on neighbor-joining, maximum parsimony and maximum likelihood methods that accords to the general rule of Pleuronectiformes evolution. But they also resulted in some confusion. Unlike data from morphological characters, P. olivaceus clustered with K.bicoloratus, but P. cinnamomeus did not cluster with P. olivaceus, which is worth further studying.

关键词

mtDNA, 16s rRNA gene sequences, phylogeny, Pleuronectiformes

Key words

mtDNA, 16s rRNA gene sequences, phylogeny, Pleuronectiformes

分类

海洋科学

引用本文复制引用

..Preliminary study on mitochondrial 16S rRNA gene sequences and phylogeny of flatfishes (Pleuronectiformes)[J].中国海洋湖沼学报(英文版),2005,23(3):335-339,5.

基金项目

Supported by Natural Science Fund of China (No. 30271036) and Natural Science Fund of Shandong Province of China (No. 30271036)

中国海洋湖沼学报(英文版)

OACSCD

2096-5508

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