首页|期刊导航|中国海洋湖沼学报（英文版）|Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome
中国海洋湖沼学报（英文版）2009，Vol.27Issue(2)：337-341,5.DOI:10.1007/s00343-008-0108-2
Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome
Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome
摘要
Abstract
An open reading frame (lcn61) of iymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector.Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene.关键词
Lymphocystis disease virus China (LCDV-cn)/ lcn61 gene/ zinc-finger protein/ prokaryotic expression/ sequence and motif analysisKey words
Lymphocystis disease virus China (LCDV-cn)/ lcn61 gene/ zinc-finger protein/ prokaryotic expression/ sequence and motif analysis分类
天文与地球科学引用本文复制引用
YAN Xiuying,SUN Xiuqin..Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome[J].中国海洋湖沼学报（英文版）,2009,27(2):337-341,5.基金项目
Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309) （863 Program, No. 2006AA100309）
