动物学研究2005，Vol.26Issue(4)：337-343,7.
圆斑蝰蛇毒七个C-型凝集素样蛋白亚基的cDNA克隆与序列分析
Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C-type Lectin-like Protein Subunits from Daboia russellii siamensis
钟树荣 1金扬 2李东升 1王婉瑜 1熊郁良1
作者信息
- 1. 中国科学院昆明动物研究所,动物毒素室,云南,昆明,650223
- 2. 中国科学院研究生院,北京,100039
- 折叠
摘要
Abstract
Total mRNA was extracted from a venom gland of snake Daboia russellii siamensis following the manufacturers protocol of the PolyATtract System 1000 kit purchased from Promega Biotech. cDNAs encoding C-type lectins were amplified by RT-PCR and subcloned into a pMD18-T vector. Fourteen positive clones were selected for nucleotide sequencing and seven cDNAs encoding various snake venom C-type lectin-like protein precursors, designated as DRS-L1, DRS-L2, DRS-L3, DRS-L4 DRS-L5, DRS-L6 and DRS-L7, were obtained. Amino acid sequences of these proteins were deduced and each contains a carbohydrate recognition domain. Of all the deduced protein sequences, only DRS-L1 seemed to represent a closer sequence similarity to α subunits of other known snake venom C-type lectin-like proteins using the BLAST program. Homology comparison combined with analysis of cysteine position indicate that DRS-L1 and DRS-L2 are probably the light chain LC2 and LC1 of factor Ⅹ activator from Daboia russellii siamensis venom, respectively. DRS-L3 and DRS-L4 might be the β subunits of higher molecular weight C-type lectin-like proteins while DRS-L5 and DRS-L6 might be β subunits of lower molecular weight C-type lectin-like proteins. DRS-L7 might be the β subunit of a platelet membrane glycoprotein Ib-binding protein similar to echicetin.关键词
圆斑蝰蛇/蛇毒C-型真凝集素/蛇毒C-型凝集素样蛋白/cDNA/序列分析Key words
Daboia russellii siamensis/SV-CTTLs/SV-CTLPs/cDNA/Sequence analysis分类
生物科学引用本文复制引用
钟树荣,金扬,李东升,王婉瑜,熊郁良..圆斑蝰蛇毒七个C-型凝集素样蛋白亚基的cDNA克隆与序列分析[J].动物学研究,2005,26(4):337-343,7.
