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1株新的新城疫病毒毒株生物学特性研究及其HN基因序列测定

李燕 王恩秀 陈溥言

中国兽医杂志2001,Vol.37Issue(6):3-4,2.
中国兽医杂志2001,Vol.37Issue(6):3-4,2.

1株新的新城疫病毒毒株生物学特性研究及其HN基因序列测定

Biological characteristics and sequencing of HN gene of NDV isolate

李燕 1王恩秀 2陈溥言1

作者信息

  • 1. 南京农业大学
  • 2. 中国科学院微生物所分子病毒室,
  • 折叠

摘要

Abstract

One virus isolate was obtained from a chicken flock with high morbidity and mortality.The isolate was confirmed as Newcastle disease virus.The biological tests proved it as a virulent strain,but the HA titers were as high as 28~10.The HN gene of the isolate was amplified by the Reverse Transcription-Polymerase Chain Reaction(RT-PCR)technique using a pair of specific primers.Then the whole HN gene was sequenced and the amino acid sequence was obtained from it.

关键词

NDV/生物学特性/HN基因/RT-PCR/序列测定

分类

农业科技

引用本文复制引用

李燕,王恩秀,陈溥言..1株新的新城疫病毒毒株生物学特性研究及其HN基因序列测定[J].中国兽医杂志,2001,37(6):3-4,2.

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