中国临床康复2004，Vol.8Issue(17)：3402-3403,2.
人类去细胞同种异体神经移植物化学萃取方法的研究
Chemical extraction of human acellular nerve allograft
摘要
Abstract
BACKGROUND: It was reported that chemical extraction could deprive nerve fiber of cells and myelin sheath. The nerve allograft made in this way did not induce host immunological rejection. However, this method has only been successful in little aninals and inferior mammals. There is still a long way to go for clinical use.OBJECTIVE: To deprive human peripheral nerve of cell and myelin sheath to get long and thick acellular nerve allograft.DESIGN: A non-random and uncontrolled experiment was conducted.SETTING and PARTICIPANTS: The experiment was conducted in the Department of Orthopaedics, the 304 Hospital of PLA. The subject was a man of 26 years old, weighing 75 kg who died of acute brain injury. The donation permission was guaranteed by his family mambers.METHODS: The ulnar nerve was taken ont and treated with Triton X-100and deoxycholate sodium of certain concentration in certain procedures. To observe cells, myelin sheath and basement membrane, the extracted nerve and normal nerve were stained in the following methods respectively: HE staining, myelin sheath staining and cellulose staining. Immunohistochemical staining was applied to display basal lamina of Schwann cells. The ultrastructure was observed with transmission electron microscope.MAIN OUTCOME MEASURES: The physical characters of acellular nerve, histological observation and ultrastructure.RESULTS: The acellular nerve showed good ductility and elasticity of perineurium. Basement membrane and basal lamina of Schwann cells were retained while cells and myelin sheath were totally cleared off. The acellular nerve was an empty matrix nerve duct without myelin sheath or its fragments.关键词
人/尺神经/周围神经/神经移植/方法Key words
When treated with Triton X-100 and deoxycholate sodium,cells and myelin sheath can be effectively cleared from human peripheral nerve, thus leaving a long and thick acellular nerve. The nerve allograft maintains the reticular columnar structure and retain分类
医药卫生引用本文复制引用
衷鸿宾,侯树勋,徐莹..人类去细胞同种异体神经移植物化学萃取方法的研究[J].中国临床康复,2004,8(17):3402-3403,2.基金项目
首都医学发展基金(2002-3049) （2002-3049）
