昆虫学报2008，Vol.51Issue(3)：342-348,7.
小地老虎几丁质酶基因的克隆与序列分析
Molecular cloning and sequence analysis of chitinase cDNA from the cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae)
摘要
Abstract
Insect chitinases have been considered as potential insecticidal molecules for development of insect control strategies. Total RNA was isolated from the midgut of the cutworm, Agrotis ipsilon (Hufnagel) prepupae. The cDNA sequence was cloned by rapid amplification of cDNA ends (RACE). The cDNA, 2 823 base pairs in length, contained an open reading frame of 1 674 base pairs coding a polypeptide of 558 amino acid residues with a predicted molecular weight of 62.5 kDa and pI 5.12. The deduced amino acid sequence contained two putative N-glyeosylation sites and twenty putative O-glycosylation sites, and two conserved domains in chitinases, i.e. the highly conserved catalytic domain at the N-terminus and the chitin-binding type 2 domain at the C-terminus. The predicted protein shared extensive similarities with chitinases from other insects, especially with the lepidopteran insects. The sequence has been deposited with GenBank under accession no. EU035316.关键词
小地老虎/几丁质酶/克隆/序列分析Key words
Agrotis ipsilon (Hufnagel)/ chitinase/ cloning/ sequence analysis分类
生物科学引用本文复制引用
朴冬花,姚磊,王玲,樊东..小地老虎几丁质酶基因的克隆与序列分析[J].昆虫学报,2008,51(3):342-348,7.基金项目
黑龙江省自然科学基金项目(C2007-7) （C2007-7）
黑龙江省教育厅科学技术研究项目(11521020) （11521020）
