青岛大学医学院学报2009，Vol.45Issue(4)：343-346,4.
冬枣提取物对S180荷瘤小鼠的免疫及抗氧化作用
EFFECT OF DONGZAO JUJUBE EXTRACT ON IMMUNE AND ANTIOXIDANTION IN S180 TUMOR-BEARING MICE
胡迎芬 1马莉 1马爱国 1梁惠 1孙永叶 1将正尧1
作者信息
摘要
Abstract
Objective To study the effects of Dongzao Jujube Extract (DJE) on immune function and antioxidation in S180-bearing mice.Methods This study consisted of 50 Kunming mice. Ten mice served as normal control (NC) group. The other 40 received subcutaneous inoculation on the left axilla of 0.2 Ml normal saline containing tumor cells, and randomized to four groups as tumor control (TC), Ethanol Extraction of Dongzao Jujube (EED), Ethyl Acetate Extraction of Dongzao Jujube (EAED) and Cyclophosphamide (CTX) group. The mice in the NC and TC groups were given saline solution by gavage once a day; those in EED and EAED groups, 100 mg/kg EED and EAED, respectively; those in CTX group were intraperitoneally injected with 20 mg/kg CTX every other day. The experiment lasted for 10 days. The mice were weighed after 24 hours of the final medication, blood samples collected from eye sockets for detection of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD). Serum interleukin (IL)-2 was determined by sandwich ELISA. The tumor, thymus gland and spleen were collected and oncostatic rate, thymus index and spleen index calculated. Results DJE significantly inhibit the growth of S180 tumor in mice, with the maximal tumor-inhibition rate reaching 24.8%. The differences of thymus and spleen index between DJE and TC groups were not significant (P>0.05), but apparently higher than that in CTX group (F=3.912,8.924;q=2.502-4.654;P<0.05). The serum level of IL-2 and SOD activity in DJE groups were significantly higher than that in the TC (F=2.932,41.299;q=2.839-12.222;P<0.05), but the level of MDA was significantly lower (F=12.917;q=5.812,3.196;P<0.05).Conclusion DJE possesses effects of inhibiting the growth of sarcoma S180, improving the immune function and strengthening antioxidation defence system in tumor-bearing mice.关键词
冬枣/白细胞介素-2/超氧化物歧化酶/丙二醛/肉瘤/小鼠Key words
Dongzao Jujubes/Interleukin-2/Superoxide dismutase/Malondialdehyde/Sarcoma/Mice分类
医药卫生引用本文复制引用
胡迎芬,马莉,马爱国,梁惠,孙永叶,将正尧..冬枣提取物对S180荷瘤小鼠的免疫及抗氧化作用[J].青岛大学医学院学报,2009,45(4):343-346,4.
