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The Influence of Endogenous Hormones on Culture Capability of Different Explants in Rice

WANG Xiu-hong SHI Xiang-yuan WU Xian-jun WANG Xu-dong ZHOU Kai-da

中国农业科学(英文版)2005,Vol.4Issue(5):343-347,5.
中国农业科学(英文版)2005,Vol.4Issue(5):343-347,5.

The Influence of Endogenous Hormones on Culture Capability of Different Explants in Rice

The Influence of Endogenous Hormones on Culture Capability of Different Explants in Rice

WANG Xiu-hong 1SHI Xiang-yuan 2WU Xian-jun 1WANG Xu-dong 2ZHOU Kai-da2

作者信息

  • 1. Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, P.R. China
  • 2. Zone of Shanxi Agriculture and Advanced Technology, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, P.R. China
  • 折叠

摘要

Abstract

The endogenous hormones (EHs) content of different explants (anther, young panicle, young embryo and mature embryo)and calli with different culture capability were analyzed by means of high performance liquid chromatography (HPLC). The results showed that the contents and ratio of endogenous hormones were one of the key factors affecting callus induction frequencies (CIF) and green plantlet differentiation frequencies (GPDF). The influence of endogenous hormones of different explants on CIF represented as: Zoatin ribosile (ZR) showed negative effect, indole-3-acetic acid (IAA) did positive effect, and gibberellic acid (GA) did negative effect except for mature embryos. The influence of endogenous hormones on green plantlet differentiation frequency (GPDF) showed: IAA and GA were negative effect; abscisic acid (ABA) and zeatin+ zeatin riboside (Z+ZR) were positive effect. The mixture ratio of endogenous hormones played a role on CIF and GPDF. IAA/Z+ZR had a positive effect on CIF, and there was a notable positive correlation between Z+ZR/IAA and GPDF, so was between ABA/IAA and GPDF.

关键词

Rice/Endogenous hormones (EHs)/Explant/Callus induction frequency (CIF)/Green plantlet differentiationfrequency (GPDF)

Key words

Rice/Endogenous hormones (EHs)/Explant/Callus induction frequency (CIF)/Green plantlet differentiationfrequency (GPDF)

分类

农业科技

引用本文复制引用

WANG Xiu-hong,SHI Xiang-yuan,WU Xian-jun,WANG Xu-dong,ZHOU Kai-da..The Influence of Endogenous Hormones on Culture Capability of Different Explants in Rice[J].中国农业科学(英文版),2005,4(5):343-347,5.

基金项目

This work was sponsored by the Sichuan Rice Breeding Project, Sichuan Youth Science Funds and Ministry of Education of China. ()

中国农业科学(英文版)

2095-3119

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