中国现代医学杂志2005，Vol.15Issue(3)：343-348,6.
甲强龙对出血性休克大白鼠的影响
Effect of methylprednisolone on hemorrhagic shock in rats
王钢 1杨晓临 2崔泽实 3关福兰2
作者信息
- 1. 中国医科大学附属第一医院,急诊科,辽宁,沈阳,110001
- 2. 中国医科大学基础医学院,辽宁,沈阳,110001
- 3. 中国医科大学实验技术中心,辽宁,沈阳,110001
- 折叠
摘要
Abstract
Objective: To investigate the effect of methylprednisolone (MP) on hemorrhagic shock in rats and elucidate the underlying mechanisms. Method: Wistar rats were bled to induce the shock and subsequently resuscitated with shed blood and normal saline. Just prior to resuscitation, the rats were divided into three groups: sham group, shock group and shock+MP groups. Results: 72 h after resuscitation, the survival rate of shock group decreased to 20%, while the survival rate of shock+MP group was 80%, the difference was significant (P ＜0.01). 18 h after resuscitation, pathologic alterations of organs showed: in shock group, pulmonary edema, leukocyte infiltration in interstitial tissue and cellular degeneration occurred. MP reduced these pathological alterations significantly. 18 h after resuscitation, creatine phosphokinase, transaminases and creatinin were elevated significantly in shock group,while these in shock+MP group were elevated slightly, the differences were significant (P ＜0.01). Increases in intracellular calcium and production of TNF-α by isolated kupffer cells stimulated by endotoxin were elevated significantly by hemorrhagic shock, which were totally prevented by MP (P ＜0.01). Conclusion: Methylprednisolone reduces organ injury and mortality caused by hemorrhagic shock by blocking increase of intracellular calcium, activation and production of TNF-α of kupffer cells and preventing systemic progressive inflammation responses.关键词
出血性休克/甲强龙/枯否细胞/细胞内钙/肿瘤坏死因子-αKey words
hemorrhagic shock/methylprednisolone/kupffer cell/intracellular calcium/TNF-α分类
医药卫生引用本文复制引用
王钢,杨晓临,崔泽实,关福兰..甲强龙对出血性休克大白鼠的影响[J].中国现代医学杂志,2005,15(3):343-348,6.
