高等学校化学学报2001，Vol.22Issue(1)：34-37,4.
量子点标记天花粉蛋白的研究
Studies on Quantum Dots-labeled Trichosanthin
张春阳 1马辉 1丁尧 1金雷 1陈瓞延 1苗琦 2聂书明3
作者信息
- 1. 清华大学物理系
- 2. 中国科学院生物物理研究所
- 3. 印第安那大学化学系,
- 折叠
摘要
Abstract
Semiconductor quantum dots were used for the first time to label trichosanthin.The absorption spectrum,fluorescent spectrum and enzymatic activity of quantum dots-labeled trichosanthin were studied in this paper.The absorbane of QDs-TCS varied to a less extent with wavelength in the range of 400－600 nm.The fluorescent spectrum of QDs-TCS underwent a blue-shift in the emission peak,but the intrinsic spectral width was unchanged.No changes were found in the enzymatic activity of QDs-TCS in comparison with trichosanthin.Distribution of QDs-TCS within human choriocarcinoma cells was simultaneously observed with two-photon laser scanning microscopy and confocal laser scanning microscopy.The results indicated that QDs-TCS could enter the cytoplasm across the membrane and aggregated around the nuleolus.关键词
量子点/天花粉蛋白/酶活性分类
化学化工引用本文复制引用
张春阳,马辉,丁尧,金雷,陈瓞延,苗琦,聂书明..量子点标记天花粉蛋白的研究[J].高等学校化学学报,2001,22(1):34-37,4.
