中国临床康复2002，Vol.6Issue(22)：3446-3447,2.
内皮素在大鼠实验性脑震荡脑组织中表达研究
Study on expression of endothelin in experimental cerebral concussion in rats
彭瑞云 1高亚兵 1王德文 1肖兴义 2陈浩宇 1吴小红 1刘杰 1胡文华 1蔡宝仁 2薛官生 2张援平 2尹晓梅2
作者信息
- 1. Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,China
- 2. The 205th Hospital of PLA,Jinzhou 121000,China
- 折叠
摘要
Abstract
Objective To study changes and significance of endothelin(ET) in rat cerebral concussion.Methods 80 Wistar male rats were used for animal model of cerebral concussion,which were sacrificed on 1,3,7,14 and 30 days after injury and the brain tissue were taken off. The expression of ET was studied in the course of cerebral concussion by means of immunohistochemistry.Results Typical clinical manifestation was observed in the 100 g group in which the pathological changes included cerebral vascular constriction and dilatation,congestion and edema of cerebral tissue,neuronal degeneration,necrosis,and obviously decreased even disappeared Nissl bodies.Increased expression of ET was observed on the first day,the positive area was seen in the plasma of endothelial cells in cerebral cortex,hippocampus,cerebellum and thalamus.ET expression peak occurred on the 7th day,the positive area was also found in the plasma of Purkinje cells in the cerebellum.Decreased ET expression was found on 14th day and returned to normal level on the 30th day.Conclusion The main pathological changes of cerebral concussion contained blood circulation disorder,and degeneration and necrosis of substantial cells.ET was involved in the brain tissue injury during the pathological process of cerebral concussion and might be related to regulation of cerebral vascular reaction,and neuron degeneration and necrosis. 关键词
cerebral concussion/ET/immunohistochemistryKey words
cerebral concussion/ET/immunohistochemistry分类
医药卫生引用本文复制引用
彭瑞云,高亚兵,王德文,肖兴义,陈浩宇,吴小红,刘杰,胡文华,蔡宝仁,薛官生,张援平,尹晓梅..内皮素在大鼠实验性脑震荡脑组织中表达研究[J].中国临床康复,2002,6(22):3446-3447,2.
