青岛海洋大学学报(自然科学版)2001，Vol.31Issue(3)：345-350,6.
Xp11.2区域YAC OAT1中表达顺序筛选
Screening of Expressed Sequences of YAC OAT1 in Xp11.2
马洪明 1曹立民 1魏勇 2柴建华2
作者信息
- 1. 青岛海洋大学水产学院,
- 2. 复旦大学遗传学研究所
- 折叠
摘要
Abstract
To screen expressed DNA sequences harbored by YAC OAT1, which locates on Xp11.2, and to explore better means to clone new disease genes in this region, YAC OAT1 DNA was used as a probe to hybridize with the in situ lysised plaque of a fetal brain cDNA library. A second cycle of hybridization was performed to enrich the positive clones. The enriched positive clones hybridized with the YAC OAT1 and human genome DNA to eliminate the false ones or the ones that contain repetitive sequences. One expressed single copy sequence clone of YAC OAT1 was obtained. The sequencing analysis of the clone showed homology to the Human Ornithine Aminotransferase (OAT) gene.关键词
酵母人工染色体（YAC）/cDNA文库/杂交分类
生物科学引用本文复制引用
马洪明,曹立民,魏勇,柴建华..Xp11.2区域YAC OAT1中表达顺序筛选[J].青岛海洋大学学报(自然科学版),2001,31(3):345-350,6.
