中德临床肿瘤学杂志（英文版）2005，Vol.4Issue(6)：347-350,4.
转染MUC1基因树突状细胞活化的淋巴细胞对BIU-87细胞的体外杀伤作用
Lethal Effect of T Cells Activated by Dendritic Cells with MUC1 Gene Transfection on BIU-87 Cells in vitro
摘要
Abstract
Objective: To observe the lethal effect of T cells activated by dendritic cells (DCs) with MUC1gene transfection (MUC1-DCs) on BIU-87 cells in vitro, and to evaluate the possibility of dendritic cells transfected by MUC1 gene as a vaccine for bladder cancer immunotherapy. Methods: MUC1 was successfully transfected into cultured human blood-derived dendritic cell with lipofectin. The transfection efficiency and immunocompetence were detected by flow cytometry and MTT colourmetry. T lymphocytes activated by MUC1-DCs were used to kill BIU-87 cell lines and normal bladder epithelium in vitro and the killing rate was evaluated by MTT colourmetry. Results: There was significant lethal effect on the BIU-87 cells and normal bladder epithelium with T cells activated by MUC1-transfected DCs, but the lethal effect on the BIU-87 cells significantly exceeded that on normal bladder epithelium (P＜0.05). The lethal effect on BIU-87 cells with T cells activated by MUC1-transfected DCs significantly exceeded that with T cells activated by no-transfection DCs (P＜0.01). Conclusion: T cells activated by MUC1-DCs could induce killing action to BIU-87 cell lines. MUC1 gene could be used as a target for bladder cancer immunotherapy.关键词
树突状细胞/MUC1/基因转染/膀胱肿瘤Key words
dendritic cell/MUC1/transfection/bladder tumor分类
医药卫生引用本文复制引用
孙凯,周四维,罗刚,曹正国,鲁雄兵,叶章群..转染MUC1基因树突状细胞活化的淋巴细胞对BIU-87细胞的体外杀伤作用[J].中德临床肿瘤学杂志（英文版）,2005,4(6):347-350,4.基金项目
This project was supported by a grant from National Natural Sciences Foundation of China (No. 30271300). （No. 30271300）
