医学分子生物学杂志2009，Vol.6Issue(4)：347-352,6.DOI:10.3870/j.issn.1672-8009.2009.04.015
规模化蛋白质相互作用的研究技术
Large-Scale Study of Protein-Protein Interactions
摘要
Abstract
Protein-protein interaction is one of the major ways by which proteins execute functions, thus playing key roles in many cellular processes. Abnormal protein-protein interactions and disorder of protein chains lead to pathological changes. Large-scale study of protein-protein interaction has recently become an important approach in the field of functional genomics and proteomics. In this review, we focus on the general large-scale experimental techniques for identifying protein-protein interactions and introduce the commonly used protein interaction databases.关键词
酵母双杂交技术/免疫共沉淀技术/蛋白质相互作用/功能基因组/数据库Key words
yeast two-hybrid technique/co-immunoprecipitation/protein-protein interactions/functional genemo/databases分类
生物科学引用本文复制引用
唐刘君,王建,杨晓明..规模化蛋白质相互作用的研究技术[J].医学分子生物学杂志,2009,6(4):347-352,6.基金项目
国家高技术研究发展计划基金项目(863计划)(No.2006AA02A310,2004BA711A19),国家重点基础研究发展规划项目(973计划) (No.2006CB910802),国家自然科学创新群体基金(No.30621063)This work was supported by grants from the National High Technology Research and Development Program of China (863 Program) (No. 2006AA02A310, 2004BA711A19), the Special Funds for Major State Basic Research Program of China (973 Program) (No. 2006CB910802) and the National Natural Science Innovation Group Foundation of China(No.30621063) （863计划）
