中华医学杂志（英文版）2001，Vol.114Issue(4)：352-354,3.
Hepatitis B virus S gene mutants in infants infected despite immunoprophylaxis
Hepatitis B virus S gene mutants in infants infected despite immunoprophylaxis
朱启 1吕晴 1俞蕙 1段恕诚 1熊思东2
作者信息
- 1. 复旦大学医学院附属儿童医院传染科 上海 200032
- 2. 复旦大学医学院免疫教研室 上海 200032
- 折叠
摘要
Abstract
Objective To assess the correlation between hepatitis B virus(HBV) surface gene mutant infection and hepatitis B (HB) vaccination failure. Methods Using sera from 106 infants who were born to HBV carrier mothers and failed in HB immunoprophylaxis, HBV S gene was amplified by PCR, transferred to nylon membranes for Southern blots, and then hybridized with oligonucleotide probes. Eleven of non-hybridizing samples were used for DNA sequencing. Results 93.4% (99/106) of the samples were HBV DNA positive, and 30.3% (30/99) failed to hybridize with at least one of the four probes. DNA sequencing confirmed that 10 of the 11 samples had an S gene mutation with amino acid (aa) change. The identified mutants included nucleotide (nt) 546T→A(aa131N→T), nt531T→C (aa126I→T), nt491A→C (aa113T→P), nt491T→A (aa113S→T), nt533C→A (aa127P→T), nt581T→A (aa143S→T), nt636A→T (aa161Y→F), and nt679A→C (aa175L→F). The sequence in one mother-infant pair was completely the same, with mutations at aa131 and aa161. Conclusions The prevalence of HBV surface mutants is about 30% in the children failing in HB vaccination. HBV mutants can infect infants by maternal-infant transmission.关键词
乙型肝炎病毒 S基因变异 乙型肝炎疫苗Key words
hepatitis B virus/S gene mutant/hepatitis B vaccine乙型肝炎疫苗免疫失败儿童S基因变异株研究朱启 吕晴 俞蕙 段恕诚 熊思东分类
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朱启,吕晴,俞蕙,段恕诚,熊思东..Hepatitis B virus S gene mutants in infants infected despite immunoprophylaxis[J].中华医学杂志（英文版）,2001,114(4):352-354,3.
