中国现代医学杂志2005，Vol.15Issue(23)：3527-3531,5.
吲哚美辛对人结肠癌细胞BfL-1、WISP-1及PCNA表达的影响
Effect of indomethacin on the expression of Bfl-1, WISP-1 and PCNA in the colon cancer HCT116 cells
摘要
Abstract
[Objective] It has been known that non steroidal anti-inflammatorydrugs (NSAIDs) can inhibit the growth of tumors through both the COX-2 dependent and independent way. To study the effect of indomethacin on the expression of Bfl-1, WISP-1 and PCNA mRNA in the human colon cancer HCT116 cells and its COX-2 independent way to inhibit tumors. [Methods] Human colon cancer HCT116 cells were treated with different concentration of indomethacin for 48 h to obtain its IC50 (50% inhibitory concentration), then the cells was treated with IC50 for 48 h. The expression of Bfl-1, WISP-1 and PCNA mRNA was detected by real-time quantitative PCR. [Results] The IC50 of colon cancer HCT116 is 316μmol/L indomethacin. Indomethacin down-regulates mRNA expression of Bfl-1, WISP-1 and PCNA in vitro. [Conclusion] Inducing apoptosis and inhibiting proliferation contribute to the anti-tumor activity of indomethacin by the COX-2 independent way of Bfl-1, WISP-1 and PCNA.关键词
吲哚美辛/HCT116/荧光定量PCR/Bfl-1/WISP-1/PCNAKey words
indomethacin/HCT116 cells/quantitative real-time PCR/Bfl-1/WISP-1/PCNA分类
医药卫生引用本文复制引用
李新华,张桂英,王娟..吲哚美辛对人结肠癌细胞BfL-1、WISP-1及PCNA表达的影响[J].中国现代医学杂志,2005,15(23):3527-3531,5.基金项目
Nation Nature Science Foundation (No: 30271516) （No: 30271516）
