中国现代医学杂志2005，Vol.15Issue(23)：3532-3535,4.
胆囊收缩素A受体拮抗剂L-364,718在实验性胰腺再生模型中的作用
Study on effect of cholecystokinin-A receptor antagonist L-364, 718 in experimental pancreas regeneration model
劳学军 1曹明溶 1庞钊 1龚瑾1
作者信息
- 1. 暨南大学附属第一医院,普外科,广东,广州,510630
- 折叠
摘要
Abstract
[Objective] To study effect of cholecystokinin-A recaptor antagonist on experimental pancreas regeneration model. [Methods] 24 rats were divided into 3 groups, as sham operation Px(-), using CCK-RA antagonist with pancreastectomy Px+CCK-RA, pancreastectomy without using CCK-RA antagonist Px by random method.After 5 days, we detected the samples wet weight of parabiliary segment from the duodenum and cell proliferation index by microscope observation using bromodeoxyuridine (BrdU) labeling,cell proliferation index(LI) and the plasma CCK concentrations were measured. [Results] 24 rats were detected, wet weight of parabiliary segment from the duodenum in Px(-) group is (86.98±3.51) mg, Px+CCK-RA group (92.46±6.67) mg, Px group (210.72±7.39) mg,compared each group have revealed significant difference (F=772.9, P ＜0.01). In Px(-) group, the LI in acinar cells was approximately 1%, but in Px and Px+CCK-RA group are about 10% and 5%; Plasma CCK concentration of each group is Px(-) group (28.22±2.45) pg/mL, Px+CCK-RA group (53.62±4.49) pg/mL and Px group (62.85± 4.33)pg/mL by radioimmunoassay, the results diseovered significant difference (F=171.8, P ＜0.01). [Conclusions] Endogenous CCK is one of the essential factors for pancreatic regeneration; secretion of them is promoted by partial pancreatectomy. But its effect are inhibited by CCK-RA, the molecular mechanism by CCK secretion is promoted remains to be elucidated.关键词
胰腺/再生/胰腺切除Key words
pancreas/regeneration/pancreatectomy分类
医药卫生引用本文复制引用
劳学军,曹明溶,庞钊,龚瑾..胆囊收缩素A受体拮抗剂L-364,718在实验性胰腺再生模型中的作用[J].中国现代医学杂志,2005,15(23):3532-3535,4.
