安徽中医药大学学报2001，Vol.20Issue(1)：35-37,3.
941方对衰老大鼠肝线粒体酶活性的影响
EFFECTS OF 941 RECIPE ON LIVER MITOCHONDRION
ENZYME FROM AGED RATS
俞超芹 1凌昌全 1缪明永 2蔡在龙 2王学敏 2毛积芳 2赵伟康3
作者信息
- 1. 中国人民解放军第二军医大学长海医院中医科，上海 200433
- 2. 中国人民解放军第二军医大学基础医学部
- 3. 上海中医药大学生物化学教研室
- 折叠
摘要
Abstract
Objective：To study functional changes in the activity of respiratory chain enzyme of mitochondrion isolated from the liver of aged rats and the effects of 941 Recipe(941R,a traditional medicine formula)on it.Method:The liver mitochondrion were isolated from rats aged 4 months(young group),24 months(senescent group)and 941 group respectively by differential centrifugation;the determination of mitochondria population in rat hepatic cell was obtained quantitatively from measurement of mitochondria protein by a bruit method;NADH oxides (NADHOX),succinct oxides(SUCOX),and the content of cytochrome a,b,c,and c1(Cyt a,b,c and c1)were evaluated by the differential spectra(dithionite-reduced minus ferricyanide-oxidized).Result:In comparison with the young group,in senescent rats there was a decline in mitochondria population;The activities of these oxidizes,however,were significantly increased,and the content of cytochrome,especially Cyt b,c1,was markedly increased;the parameters cited above in 941R group were significantly elevated over those in young and senescent rats.Conclusion:These results suggest that the enhancement of respiratory enzymatic activities of the liver mitochondrion from aged rats appears to be some compensatory mechanism by witch increase workload of the remaining intact population of mitochondrion,and 941R more efficiently improves the compensatory mechanism.关键词
线粒体/呼吸链/延缓衰老/941方Key words
Mitochondrion/Respiratory chain enzyme/Delayed aging/941 Recipe分类
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俞超芹,凌昌全,缪明永,蔡在龙,王学敏,毛积芳,赵伟康..941方对衰老大鼠肝线粒体酶活性的影响[J].安徽中医药大学学报,2001,20(1):35-37,3.
