南京医科大学学报（英文版）2005，Vol.19Issue(1)：35-38,4.
Effects of allitridin on the expression of transcription factor T-bet mRNA of cytomegalovirus-induced myocarditis in mice
Effects of allitridin on the expression of transcription factor T-bet mRNA of cytomegalovirus-induced myocarditis in mice
Yi Xu 1Feng Fang1
作者信息
- 1. Laboratory of Pediatric Clinical Virology of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
- 折叠
摘要
Abstract
Objective: To investigate the effects of allitridin on the expression of transcription factor T-bet of cytomegalovirusinduced myocarditis in mice and to analyze its role in anti-cytomegalovirus mechanisms. Methods: Sixty mice were randomly divided into allitridin therapy group, infected controls group and bland controls group. Allitridin therapy group were given to mice via the intraperitoneal same volume of 0.89% sodium chloride and bland controls group were only given with the same volume of 0.89% sodium chloride, without infected MCMV. All experimental mice were sacrificed on day 3, 5, 7 and 14 i.p. ( n = 5 per time point). The expression levels of transcription factor T-bet were measured by RT-PCR. The expression levels of Th1 cytokine IFN-γ were measured by ELISA. Results:MCMV infection could markedly down-modulate the expression of IFN-γ and T-bet mRNA ( P ＜ 0.01). Allitridin significantly up-regulated the expression of Th1 cytokines IFN-γ and Th1-specific transcription factor T-bet mRNA ( P ＜ 0.01). Conclusion: Allitridin up-regulates the expression of transcription factor T-bet mRNA which is an important, but not the only factor to mediate the constitutive expression of Th1 cytokines IFN-γ.关键词
allitridin/cytomegalovirus/transcription factor/cytokineKey words
allitridin/cytomegalovirus/transcription factor/cytokine分类
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Yi Xu,Feng Fang..Effects of allitridin on the expression of transcription factor T-bet mRNA of cytomegalovirus-induced myocarditis in mice[J].南京医科大学学报（英文版）,2005,19(1):35-38,4.
