作物学报2000，Vol.26Issue(1)：35-39,5.
细胞质雄性不育棉花线粒体蛋白质和DNA的分析
Analyses of Mitochondrial Protein and DNA from Cytoplasmic Male Sterile Cotton
王熹 1陶龙兴 1黄效林 1俞美玉 1谭红 2李志东2
作者信息
- 1. 中国水稻研究所,浙江,杭州,310006
- 2. 中国科学院成都生物研究所,四川,成都,610041
- 折叠
摘要
Abstract
Analyses of SDS-PAGE, RAPD and RFLP for mitochondrial protein and DNA from anthers and seedlings of cytoplasmic male sterile cotton with the cytoplasm of Gossypurn harknessii L. were conducted. The results showed that the mitochondrion in the sterile anther was short of a 31 kDa protein and that the cytogenotype of sterile plant was significantly different from that of fertile plant. When five mitochondrial genes were used as probes in the RFLP analysis, one of them, cox Ⅱ, showed the absence of a 1.9 kb band when hybridized with the Hind Ⅲ digested DNA from the sterile plant. The relationship between cotton mitochondrial mutation and cytoplasmic male sterility was also discussed in this paper.关键词
棉花/细胞质雄性不育/线粒体蛋白质和DNAKey words
Cotton/Cytoplasmic male sterility/Mitochondrial protein and DNA分类
农业科技引用本文复制引用
王熹,陶龙兴,黄效林,俞美玉,谭红,李志东..细胞质雄性不育棉花线粒体蛋白质和DNA的分析[J].作物学报,2000,26(1):35-39,5.
