中华医学杂志（英文版）2001，Vol.114Issue(4)：355-359,5.
Immunotoxin depletion of T cells and its effect on hematopoietic progenitor cells in human cord blood
Immunotoxin depletion of T cells and its effect on hematopoietic progenitor cells in human cord blood
许蔓春 1吕善根 2沈倍奋 3黎燕3
作者信息
- 1. 广州军区广州总医院儿科 广州 510010
- 2. 北京解放军总医院小儿内科 北京 100853
- 3. 军事医学科学院分子免疫室 北京 100850
- 折叠
摘要
Abstract
Objective To study the selective toxicity of immunotoxin (IT) onT cells in cord blood and simultaneously determine its effect on hematopoietic progenitor cells. Methods The percentage of CD5 and CD8 T cell subsets in cord blood (CB) and bone marrow (BM) as well as peripheral blood (PB) was measured by immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). One-way mixed lymphocyte cultures (MLC) were performed to compare the proliferative response of CB with that of PB. The proliferative capability of cord blood T cells and T lymphocyte transformation capacity were evaluated in the presence of anti-CD8 or anti-CD5 immunotoxin by one-way MLC and colorimetric MTT (tetrazolium) assay, respectively. The effect of IT on the growth of hematopoietic progenitor cell of colony forming unit-granulocyte and macrophage (CFU-GM), burst forming unit-erythroid(BFU-E), multipotential hemotapoietic progenitors (CFU-Mix) from CB were estimated by colony-forming assays. Results A certain proportion of CD5 and CD8 T cells existed in CB. The alloproliferative capacity of CB was similar to that of PB. CD5: Ricin at a dosage of 1×10-10-1×10-8 mmol/L and CD8: Ricin concentration in the range of 1×10-9-1×10-8 mmol/L effectively decreased both the proliferative capability of T cells in MLC during CB and T cell transformation. Over the dosage of 1×10-10-1×10-9 mmol/L, both kinds of IT didn't obviously affect the growth of hematopoietic progenitor cells. Conclusion CD5: Ricin and CD8: Ricin may effectively deplete T cells and may not significantly inhibit the function of hemaptopoietic cells at a specific dosage.关键词
脐血 免疫毒素 T细胞去除 造血干细胞Key words
fetal blood/immunotoxin/T cell depletion/hematopoietic stemcells免疫毒素去除脐血T细胞的实验研究许蔓春 吕善根 沈倍奋 黎燕分类
医药卫生引用本文复制引用
许蔓春,吕善根,沈倍奋,黎燕..Immunotoxin depletion of T cells and its effect on hematopoietic progenitor cells in human cord blood[J].中华医学杂志（英文版）,2001,114(4):355-359,5.
