海南师范学院学报（自然科学版）2004，Vol.17Issue(4)：356-362,7.
人源抗菌肽LL-37在烟草中的表达
Expression of human antimicrobial peptide LL-37 in Nicotiana tabacum
杨应华 1符碧1
作者信息
- 1. 海南师范学院,生物系,海南,海口,571158
- 折叠
摘要
Abstract
A cDNA fragment encoding human cathelicidin LL-37 linked to a signal peptide sequence of tobacco pathogenesis-related protein 1b gene was introduced into a plant expression vector, pBin438 and stably integrated into Nicotiana tabacum cv. NC-89 genome using Agrobacterium tumefaciens-mediated transformation. The presence of the fusion cDNA in the transgenic plant was confirmed by PCR and its expression was confirmed by Western blot analysis. Protein extracts from transgenic tobacco plant leaves exhibited antibacterial activity. This is the first report of synthesis of biologically active LL-37 in plants.关键词
LL-37/抗菌肽/转基因植物Key words
antimicrobial peptide/LL-37/transgenic plant分类
生物科学引用本文复制引用
杨应华,符碧..人源抗菌肽LL-37在烟草中的表达[J].海南师范学院学报（自然科学版）,2004,17(4):356-362,7.
