中德临床肿瘤学杂志（英文版）2008，Vol.7Issue(6)：358-360,3.
胞苷脱氨酶基因对小鼠大剂量化疗的保护作用
Protection of a cytidine deaminase gene gainst toxicity of high dose chemotherapy in mice
摘要
Abstract
Objective:To explore the feasibility of transfecting cytidine deaminase(CD)gene into mouse bone marrow cells in order to observe the drug resistance of high dose Ara-C and improve the tolerance of myelosuppression following combination chemotherapy.Methods:Human cytidine deaminase gene was transfected into mice bone marrow cells by retroviral vector.Resistant colony-forming unit granulocyte-macrophage(CFU-GM)assay was performed after the transfected mice bone marrow cells treated by the Ara-C.DNA was extracted from mice bone marrow cells.The drug resistant gene in mice bone marrow cells after transfection was detected by PCR.Results:Bone marrow cells of lhe donor mice cultured with lhe retroviral producer cells showed the drug resistant colonies and resistance to Ara-C,so did accept mice transplanted with the CD gene(CFU-GM of donor mice was 52%,X2=124.62,P＜0.01:accept mice was 54%,X2=126.26.P＜0.01,both compared with the contrast group).The animal survival rate was significantly higher in gene transfected group than that of the control(X2=7.42.P＜0.01).CD gene of transfected bone marrow cells was confirmed by PCR.Conclusion:CD gene can be transfected into bone marrow cells of mice efficiently and increase the drug resistance to Ara-C.关键词
cytidine deaminase/gene therapy/Ara-CKey words
cytidine deaminase/gene therapy/Ara-C分类
临床医学引用本文复制引用
Bo Chen,Caigang Liu,Yang Lu,Ping Lu,Feng Jin,Fan Yao,Ruishan Zhang,Jinlong Liu,Shicheng Zhao..胞苷脱氨酶基因对小鼠大剂量化疗的保护作用[J].中德临床肿瘤学杂志（英文版）,2008,7(6):358-360,3.基金项目
a grant fnom the National Natural Science Foundation of China(No.30471678). （No.30471678）
