中国药科大学学报2008，Vol.39Issue(4)：358-364,7.
氧化/还原修饰肝素对高糖诱导肾小球系膜细胞凋亡及其信号转导途径的影响
Influence of OR-heparin,a chemically modified heparin,on high glucose-induced mesangial cell apoptosis and its signal transduction pathway
摘要
Abstract
To investigate whether chemically modified non-anticoagulation heparin derivate (periodate oxidation/borohydride reduction-modified heparin (OR-heparin)) could inhibit high glucose-induced human mesangial cell apoptosis and to explore its possible mechanism.Methods:Mesangial cells were exposed to high glucose or high glucose with OR-heparin for 24 h.Apoptosis was evaluated by Hoechst 33258 staining and Fluorescent activated cell sorting analysis.The expressions of apoptosis-related proteins ( p53,Bcl-2,Bax,cytosolic cytochrome C) were determined by Western blotting.NF-kB translocation was observed under fluorescence microscopy by using Cy3-1abeled antibody.Caspase-3 activity was detected by colorimetry.Results:OR-heparin significantly inhibited high glucose-induced mesangial cell apoptosis and the expression of apoptosis-related proteins.It also blocked NF-kB translocation induced by high glucose.Conclusion:OR-heparin inhibits high glucose-induced mesangial cell apoptosis through inhibiting the expression of apoptosis-related proteins and it may be due to the blockage of the translocation of NF-kB.关键词
高碘酸氧化/硼氢化钠还原修饰肝素/肾小球系膜细胞/凋亡/高糖Key words
OR-heparin/mesangial cell/apoptosis/high glucose分类
医药卫生引用本文复制引用
尹登科,姚文兵,高向东..氧化/还原修饰肝素对高糖诱导肾小球系膜细胞凋亡及其信号转导途径的影响[J].中国药科大学学报,2008,39(4):358-364,7.基金项目
This study was supported by the National Natural Science Foundation(No.30472061.,,No.306724709)and the Teaching and Research Award Program for Outstanding Young Teachers(No.2002383) （No.30472061.,,No.306724709）
