中山大学学报（医学科学版）2009，Vol.30Issue(4)：361-366,6.
最优化细胞因子诱导杀伤细胞/自然杀伤细胞少因子培养体系的探索
Exploratory Research of Setting an Optimal Minor-factor Culture System of Cytokine-induced Killer Cells/ Natural Killer Cells
摘要
Abstract
[Objective] In order to explore the optimal minor-factor culture system of CIK/NK cells by proliferating CIK/NK cells using single-factor,bi-factor,and tri-factor combinations of IL-2,IL-7,and IL-12.[Methods] Ficoll-Hypaque method was used to separate cord blood mononuclear cells and divide them into 6 groups.Add single-factor,bi-factor,and tri-factor combinations (IL-7;IL-12;IL-7 + IL-12;IL-2 + IL-7;IL-2 + IL-12;IL-2 + IL-7 + IL-12) of IL-2 (80 ng/mL),IL-7 (40 ng/mL),and IL-12 (40 ng/mL) and culture them in total 21 days then harvest the cells.To collect cell suspensions of each group in culture outset and day 21 and to count the proportion of CD3+ CD56+ CIK cells and CD3- CD56+ NK cells with flow cytometry.[Results] The proportion of CIK cells in single-factor culture system of IL-12 was the highest in all groups (30.23 ± 1.18%).The proportion of CIK cells in bi-factor culture system of IL-2 + IL-12 can raised to 18.58 ± 0.68%.The proportion of CIK cells of the two groups above can reach the level of that using traditional multi-factor culture methods.NK cell proportion in IL-12 was 30.23 ± 1.18%,NK cell proportion in IL-2 + IL-7 can also reach to 29.52 ± 0.89%.[Conclusions] It is adoptable to proliferate CIK/NK cells using minor-factor culture system of IL-12,IL-2 + IL-12,or IL-2 + IL-7.关键词
细胞培养/细胞因子诱导杀伤细胞/自然杀伤细胞/细胞因子Key words
cell culture/cytokine-induced killer cell/natural killer cell/cytokine分类
生物科学引用本文复制引用
吴燕峰,林永潮,黎阳,王潇娉,魏菁..最优化细胞因子诱导杀伤细胞/自然杀伤细胞少因子培养体系的探索[J].中山大学学报（医学科学版）,2009,30(4):361-366,6.基金项目
国家自然科学基金(30500469) （30500469）
广东省科技计划项目(2008B030301066) （2008B030301066）
广州科学仪器协作共用网基金(2006034) （2006034）
中山大学北校区学生科研基金(2006031) （2006031）
