水产学报2007，Vol.31Issue(3)：361-368,8.
鳜和鲢肌肉蛋白质氨基酸组成及其蛋白质组成的比较分析
Comparative analysis of the amino acid composition and proteomic patterns of the muscle proteins from two teleosts, Siniperca chuatsi L. and Hypophthalmichthys molitrix L.
摘要
Abstract
The amino acid composition and proteomic pattern of the muscle proteins from two teleosts, the mandarin fish, Siniperca chuatsi and silver carp, Hypophthalmichthys molitrix were analyzed by HPLC and two-dimensional (2-D) gel electrophoresis. The total amount of essential amino acids in S. chuatsi is 34.7 mg·g-1 of the muscle protein, while that in H. molitrix is 33.6 mg·g-1. The values for the taste-enhancer amino acids (aspartic acid and glutamic acid) are 23.8 mg·g-1 in S. chuatsi and 15.3 mg·g-1 in H. molitrix. The muscle proteins from the two types of fish were resolved into a similar number of spots in 2-D gels (about 260 at pI 3-10), although the protein-size distributions are dramatically different between the two teleosts. Our study is the first to demonstrate significant differences in protein composition and patterns between two species of farmed fish, and provides reference values for aquaculture and efforts aimed at fish stock improvement and fish protein utilization.关键词
氨基酸/蛋白质组成/二维电泳/鳜/鲢Key words
amino acid/proteomic pattern/2-D gel electrophoresis/Siniperca chuatsi/Hypophthalmichthys molitrix分类
农业科技引用本文复制引用
唐建洲,张东裔,成嘉,刘芳,符贵红,张建社..鳜和鲢肌肉蛋白质氨基酸组成及其蛋白质组成的比较分析[J].水产学报,2007,31(3):361-368,8.基金项目
NSFC (30571414), Hunan NSF (05JJ30151 ) and Changsha City (K051127-72) （30571414）
国家自然科学基金(30571414) （30571414）
湖南省自然科学基金(05JJ30151) （05JJ30151）
长沙市科技局重点创业基金(K051127-72) （K051127-72）
