中国药科大学学报2004，Vol.35Issue(1)：36-39,4.
人血浆中盐酸多奈哌齐的HPLC-MS测定法
Quantitation of Donepezil in Human Plasma by HPLC-MS
丁黎 1郝歆愚 1李丽敏 1卞晓洁 1张胜强1
作者信息
- 1. 中国药科大学药物分析教研室,南京,210009
- 折叠
摘要
Abstract
AIM and METHOD:A highly sensitive method for quantitation of donepezil in human plasma was established by using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS),with phenopromlamine hydrochloride as the internal standard (I.S.).After alkalizing with saturated sodium bicarbonate,plasma was extracted with ethyl acetate and the extracts were separated by HPLC on a C18 reversed-phase column with methanol-water-acetic acid-triethylamine (70:30:0.3 :0.3,v/v ) as mobile phase.LC-ESI-MS was performed in the selected ion monitoring (SIM) mode using target ions at m/z 380 for donepezil and m/z 344 for the I.S..RESULT:Calibration curves,which were linear over the range of 0.2 to 20 ng/ ml,were established contemporaneously with the analyses of each batch of samples,along with low (0.2 ng/ml),medium (2 ng/ml) and high (20 rng/ml) quality control samples.The intra and inter-assay variability ranged from 1.25% to 9.84% for the low,medium and high quality control samples.The extraction recovery of donepezil from plasma was in the range of 87.5% ～ 91.9%.CONCLUSION:The method has been used successfully to study donepezil pharmacokinetics in human plasma.关键词
盐酸多奈哌齐/HPLC-MS/血浆/含量测定Key words
Donepezil/HPLC-MS/Human plasma/Determination of content分类
医药卫生引用本文复制引用
丁黎,郝歆愚,李丽敏,卞晓洁,张胜强..人血浆中盐酸多奈哌齐的HPLC-MS测定法[J].中国药科大学学报,2004,35(1):36-39,4.
