滑膜肉瘤融合基因SYT-SSX检测OA
Detection of the SYT-SSX Fusion Gene in Synovial Sarcoma
目的:研究采用逆转录-聚合酶链反应的方法在滑膜肉瘤石蜡包埋组织中检测SYT-SSX融合基因的可行性和临床意义.方法:收集20例滑膜肉瘤标本,均为甲醛固定,石蜡包埋组织.SYT-SSX融合基因采用逆转录-聚合酶链反应(RT-PCR)检测.看家基因PBGD作为内参照.结果:所有标本均可检测到PBGDmRNA的表达.18例检测到融合基因SYT-SSX的表达,其中,SYT-SSX1型占12例,SYT-SSX2型占6例(4例为单相型滑膜肉瘤).结论:在滑膜…查看全部>>
Objective: To investigate the feasibility and significance of detecting SYT-SSX fusion gene in paraffin-embedded tissues of synovial sarcoma (SS) by reverse-transcriptase polymerase chain reaction(RT-PCR) methods. Methods: Twenty cases of SS tumors from archival materials were collected and all samples were formalin-fixed and paraffin-embedded (FFPE). SYT-SSX fusion transcript was detected by RT-PCR. Home-keeping gene Porphobilinogen Deaminase (PBGD) was reg…查看全部>>
杨翎;范钦和;张炜明
南京医科大学第一附属医院病理科,南京,210029,中国南京医科大学第一附属医院病理科,南京,210029,中国南京医科大学第一附属医院病理科,南京,210029,中国
基础医学
滑膜肉瘤SYT-SSX融合基因逆转录-聚合酶链反应
synovial sarcomaSYT-SSX fusion geneRT-PCR
《南京医科大学学报(英文版)》 2004 (1)
36-39,4
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