南京医科大学学报(英文版)2004，Vol.18Issue(1)：36-39,4.
滑膜肉瘤融合基因SYT-SSX检测
Detection of the SYT-SSX Fusion Gene in Synovial Sarcoma
杨翎 1范钦和 1张炜明1
作者信息
- 1. 南京医科大学第一附属医院病理科,南京,210029,中国
- 折叠
摘要
Abstract
Objective: To investigate the feasibility and significance of detecting SYT-SSX fusion gene in paraffin-embedded tissues of synovial sarcoma (SS) by reverse-transcriptase polymerase chain reaction(RT-PCR) methods. Methods: Twenty cases of SS tumors from archival materials were collected and all samples were formalin-fixed and paraffin-embedded (FFPE). SYT-SSX fusion transcript was detected by RT-PCR. Home-keeping gene Porphobilinogen Deaminase (PBGD) was regarded as intemal control. Results: PBGD mRNA was detected in all 20 tumor cases (100%). SYT-SSX fusion transcript was detected in 18 tumor cases (90%). In 18 SYT-SSX positive SS cases, there are 12 present SYT-SSX1 fusion transcript and 6 present SYT-SSX2 fusion transcript. SYT-SSX1 fusion transcript can be seen in 9 monophasic SS and 3 biphasic SS. In 6 SYT- SSX2 positive SS cases, 4 were monophasic SS and 2 were biphasic. Conclusion: Detection of SYT-SSX fusion transcripts in FFPE tissues for diagnosis of SS is feasible and sensitive. Subtypes of SYT-SSX fusion gene may provide prognosis information.关键词
滑膜肉瘤/SYT-SSX融合基因/逆转录-聚合酶链反应Key words
synovial sarcoma/SYT-SSX fusion gene/RT-PCR分类
医药卫生引用本文复制引用
杨翎,范钦和,张炜明..滑膜肉瘤融合基因SYT-SSX检测[J].南京医科大学学报(英文版),2004,18(1):36-39,4.
