天然产物研究与开发2007，Vol.19Issue(3)：369-373,5.
超滤分离和鉴定三种香菇多糖
Isolation and Identification of Three Lentinans Obtained by Ultrafiltration from Lentinus edodes
摘要
Abstract
The Lentinan was extracted by hot water from the fruits bodies of Lentinus edodes,and separated by two kinds of ultrafiltration membranes to yield three water soluble polysaccharides Le1,Le2 and Le3. They are consisted of two components. Their molecular weight was determined using gel-filtration chromatography. The 13C NMR and IR spectra confirmed that the sugar residue of Le1 was α-glycosidically linked, the sugar residue of Le3 was β-glycosidically linked. The polysaccharides component were determined by gas chromatography, three lentinans were composed of glucose (Glu), arabinose (Ara), xylose (Xyl), mannose (Man) and galactose (Gla). The neutral saccharide molar ratios Ara: Xyl: Man: Gal: Glu are as follows: Le1,0.15∶0.52∶1.00∶1.20∶7.20; Le2, 0.21∶0.68∶1.00∶1.02∶11.56; Le3, 0.29∶0.42∶1.00∶0.85∶16.20. The average molecular weight (Mw) of the three polysaccharides Le1, Le2 and Le3 were determined to be 4.02×104, 2.16×105, 8.93×105, respectively.关键词
多糖/超滤/分子量/组成/结构性质Key words
Lentinan/ultrafiltration/molecular weight/component/structure property分类
医药卫生引用本文复制引用
谢红旗,周春山,杜邵龙,周尽花,汤建萍..超滤分离和鉴定三种香菇多糖[J].天然产物研究与开发,2007,19(3):369-373,5.基金项目
This work was supported by National Middle and Small Enterprise Innovation Foundation(02C26214300675) （02C26214300675）
