动物学研究2009，Vol.30Issue(4)：369-376,8.DOI:10.3724/SP.J.1141.2009.04369
中华大蟾蜍Mest基因的cDNA克隆和表达分析
cDNA Cloning and Expression Analysis of Mest Gene in the Bufo gargarizans
摘要
Abstract
The Mest (mesoderm-specific transcript) gene has been considered an imprinting gene in human and mouse, and was also confirmed in other mammals and flowering plants. To investigate the function and evolution of this gene, the cDNA of full length Mest gene was obtained using 5'- and 3'-RACE from the Chinese Large Toad (Bufo gargarizans). The transcript is 1325bp in length which contains a complete open reading frame (ORF) encoding a polypeptide of 326 amino acids (GenBank accession number: ABQ10905). There is a typical α/β hydrolase fold domain in the putative gene product, and it shows high similarity to sequence of homologous protein of Xenopus tropicali (86%), mammlian (70% - 80%). RT-PCR (reverse transcriptase-polymerase chain reaction) analysis demonstrated that the Bufo gargarizans Mest (BgMest) gene is expressed widely in testis, ovary, liver, kidney, spleen, brain, stomach and lung. The conservation of the BgMest gene sequences, protein secondary structure of the BgMest protein, in addition to the expression pattern of the BgMest gene, suggested that the function of BgMest was conserved in amphibians. However, the phylogenetic tree of the imprinting gene of the mammals and other vertebrates examined in this study indicated their divergent origins.关键词
中华大蟾蜍/Mest基因/印记基因/RACE/RT-PCRKey words
Bufo gargarizans/Mest gene/Imprinting gene/RACE/RT-PCR分类
生物科学引用本文复制引用
王晶晶,聂刘旺,贾瑞,汪宁..中华大蟾蜍Mest基因的cDNA克隆和表达分析[J].动物学研究,2009,30(4):369-376,8.基金项目
Foundation items: Supported by the National Natural Science Foundation of China (No.30770296) （No.30770296）
the Natural and Science Key Project of Anhui Educational Departmem (KJ2007A022) （KJ2007A022）
the Key Lab Project of Biotic Environment and Ecology Safety in Anhui Province (2006) （2006）
