中国海洋湖沼学报（英文版）2006，Vol.24Issue(4)：370-377,8.
Evaluation of the damage in fish spermatozoa cryopreservation
Evaluation of the damage in fish spermatozoa cryopreservation
摘要
Abstract
Cryodamages occur during sperm cryopreservation. Cryopreservation of fish sperm usually results in marked decrease in sperm quality, such as swelling or disruption of the plasma membrane, mitochondrial dysfunction, diminished sperm motility, impaired velocity, shorter motility period, denaturation, and release of some enzymes from spermatozoa. In this paper, damages in morphology, physiology,biochemistry and metabolism, and genetic integrity of fish semen after cryopreservation are discussed.New approaches in assessment of fish thawed sperm quality such as computer assisted sperm analysis,flow cytometic analysis combined with fluorescent probes and single cell gel electrophoresis are also briefly reviewed.关键词
cryodamage/cryopreservation/fish sperm/freezing and thawingKey words
cryodamage/cryopreservation/fish sperm/freezing and thawing分类
生物科学引用本文复制引用
LI Jun ,LIU Qinghua ,ZHANG Shicui..Evaluation of the damage in fish spermatozoa cryopreservation[J].中国海洋湖沼学报（英文版）,2006,24(4):370-377,8.基金项目
Funded by the National High Technology R&D Program of China (863Program, Nos. 2001AA621100, 2003AA603510 and 2004AA603310). （863Program, Nos. 2001AA621100, 2003AA603510 and 2004AA603310）
