中国农业科学(英文版)2004，Vol.3Issue(5)：371-375,5.
Study on Immunochemical Assays for the Organophosphorus Insecticide Chlorpyrifos
Study on Immunochemical Assays for the Organophosphorus Insecticide Chlorpyrifos
WU Gang 1HUANG Ya-li 2ZHU Guo-nian 2WU Hui-ming 2LI Cong1
作者信息
- 1. zhejiang Entry-Exit Inspection & Quarantine Bureau, Hangzhou 310012, P.R.China
- 2. Institute of Pesticide & Environmental Toxicology, Zhejiang University, Hangzhou 310029, P.R.China
- 折叠
摘要
Abstract
The anti-chlorpyrifos polyclonal antibodies were obtained by using the artificial immune antigen to immune in New Zealand′s white rabbits. The enzyme-tagged antibodies were prepared by coupling horseradish peroxidase (HRP) to the purified antibody with the modified sodium periodate method. The indirect competitive enzyme linked immuno-sorbent assays (ELISA) and the HRP-taggedantibodydirect ELISA (E-Ab) were established, respectively.The limit of detection (LOD) for the indirect ELISA and E-Ab were 0.0033 and 0.0042 μg mL-1, respectively. The linear detection ranged well from 0.005 to 2.0 μg mL-1.关键词
Chlorpyrifos/Antibody/Immunochemical assaysKey words
Chlorpyrifos/Antibody/Immunochemical assays引用本文复制引用
WU Gang,HUANG Ya-li,ZHU Guo-nian,WU Hui-ming,LI Cong..Study on Immunochemical Assays for the Organophosphorus Insecticide Chlorpyrifos[J].中国农业科学(英文版),2004,3(5):371-375,5.
