生态学报2009，Vol.29Issue(7)：3722-3728,7.
新疆泥火山细菌遗传多样性
The bacterial diversity from mud volcano in Xinjiang by culture-independent approach
摘要
Abstract
In this study, the mixed environmental genomic DNA was isolated directly from the mud volcano soil in Usu county of Xinjiang. The 16S rRNA genes were amplified with bacterial universal primers and cloned into the pGEM-T Easy Vector to construct a16S rRNA clone library.The total 150 clones were selected by amplified rDNA restriction analysis(ARDRA) using restriction enzymes Hae Ⅲ. Of these, 16 restriction fragment length polymorphism(RFLP)types were used for sequencing. A part of these sequences were closely related to the phylum: Proteobacteria,Firmicutes,Fusobacteria and Actinobacteria (>97% sequence similarity), while another portion showed less affiliation with known taxa (<97% sequence similarity) and might represent novel taxa. The results indicated that the mud volcano in Usu county of Xinjiang is rich in microbial species,which is worth further studying.关键词
泥火山/微生物多样性/非培养方法/系统发育Key words
Mud volcano/Microbial diversity/Culture-independent method/Phylogeny分类
生物科学引用本文复制引用
马小龙,娄恺,王芸,杨红梅,王纯利,毛培宏,金湘,常玮,房世杰,张评浒..新疆泥火山细菌遗传多样性[J].生态学报,2009,29(7):3722-3728,7.基金项目
973计划前期研究专项资助项目(2008CB417214) （2008CB417214）
国家科技基础条件平台资助项目(2005DKA21201-12) （2005DKA21201-12）
新疆特殊环境微生物实验室开放课题资助项目(XJYS0203-2005-01) （XJYS0203-2005-01）
新疆农科院青年科技创新基金资助项目(2007Q07) （2007Q07）
