摘要
Abstract
BACKGROUND:Local hypothermia of the spinal cord protects against and lessens the secondary injury after the traumatic injury to the spinal cord, but whether the level of local hypothermia correlates with its extent of protection is still unclear.OBJECTIVE:To study the correlation between the extent of protection and the level of hypothermia in spinal ischemic injury in rats. DESIGN:Totally randomized controlled trials.SETTING AND SUBJECTS:This experiment was conducted at the Laboratory of the Third Division of General Surgery of China Medical University(CMU).Male Wistar rats were used in the trials.INTERVENTIONS:The spinal ischemic model of rats was prepared, and hyperthermic saline was perfused to the L5- L10 of subarachnoid cavity.The temperature of the spinal cord was maintained at four levels:38 ℃, 34 ℃, 30 ℃ and 18 ℃ ,and the proximal artery blood pressure was kept between 5.3 kPa to 6.6 kPa.The blockage time was 12 min and 16 min respectively.MAIN OUTCOME MEASURES:The number of rats with paraplegia,the index of paraplegia and histological observations.RESULTS:The number of rats with paraplegia and the index of paraplegia decreased following the decreasing temperature.The number of rats with paraplegia was 6,3,0 and 0 respectively for groups of 38 ℃, 34 ℃, 30 ℃ and 18 ℃ after blockage was applied 12 min,and the number of rats with paraplegia was 8,6,5 and 0 after blockage was applied for 16 min. The index of paraplegia was 2,1,2,3 and 0 after it was blocked for 12 min in 38 ℃ group and 4,1,2,1 and 0 in 34 ℃ group. However, in group of 30 ℃ and 18℃ ,the index of paraplegia was 0;the index of paraplegia was 0, 0,1 and 4 after it was blocked for 16 min in 38 ℃ group,2,1,3 and 0 in 34 ℃ group and 4,2,2,0 and 0 in 30 ℃ group; however, the index of paraplegia was 0 in 18℃ group. The histological examination showed that with the lowering temperature, the spinal ischemic injury was lessened.CONCLUSION:Compared with light and moderate hypothermia, deep hypothermia has a more apparent protection against the spinal ischemic injury, and there is a positive correlation between the level of local hypothermia and its extent of protection against the spinal ischemic injury.关键词
脊髓/再灌注损伤/低温/组织学分类
医药卫生
