首页|期刊导航|中华医学杂志（英文版）|Effect of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry on identifing biomarkers of endometriosis
中华医学杂志（英文版）2009，Vol.122Issue(4)：373-376,4.DOI:10.3760/cma.j.issn.0366-6999.2009.04.003
Effect of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry on identifing biomarkers of endometriosis
Effect of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry on identifing biomarkers of endometriosis
摘要
Abstract
Background Endometriosis is a common gynecological disease. This study aimed to screen proteins that were expressed differently in patients with endometriosis versus normal controls using proteomic techniques, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).Methods Protein chip SELDI-TOF-MS combines the advantages of microarray and mass spectrometry, and can screen latent markers in sera of patients with endometriosis. Serum samples from patients and normal volunteers were analyzed by SELDI-TOF-MS.Results After comparing the serum protein spectra of 36 patients with 24 normal controls, 24 differently expressed potential biomarkers (P <0.01) were identified. Using Biomarker Pattern software, we established a tree model of the 60 serum protein spectra. When using the three biomarkers to classify the samples, the sensitivity for diagnosing endometriosis was 91.7%, specificity was 95.8%, and coincidence rate was 93.3%. Then we used serum samples from 12 patients and 8 normal controls to validate the tree model and report the sensitivity for diagnosing endometriosis was 91.7%, specificity was 75%, and coincidence rate was 85%.Conclusions SELDI-TOF-MS may be a useful tool in high-risk population screening for endometriosis. The identification and application of the biomarkers need to further study.关键词
endometriosis/ proteomics/ surface-enhanced laser desorption/ionization/mass spectrometryKey words
endometriosis/ proteomics/ surface-enhanced laser desorption/ionization/mass spectrometry分类
医药卫生引用本文复制引用
ZHANG Hong ,FENG Jie ,CHANG Xiao-hong,LI Zhong-xing,WU Xiao-yi,CUI Heng..Effect of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry on identifing biomarkers of endometriosis[J].中华医学杂志（英文版）,2009,122(4):373-376,4.基金项目
This study was supported by the grants from Beijing Municipal Science & Technology Commission (No.H030930040230) and the National Natural Science Foundation of China (No.30772319). （No.H030930040230）
