中国药科大学学报2001，Vol.32Issue(1)：37-40,4.
RP-HPLC法测定穿龙薯蓣总皂甙中薯蓣皂甙元的含量
RP-HPLC Determination of Diosgenin in Total Saponin from Dioscorea Nipponica Mak
都述虎 1王晓华 1夏重道 1刘文英2
作者信息
- 1. 安徽省医学科学研究所， 合肥 230061
- 2. 中国药科大学药物分析教研室， 南京 210009
- 折叠
摘要
Abstract
AIM: To develop the method of the determination of total saponinsin Dioscorea nipponica Mak. by reversed-phase high performance liquid chromatography. METHOD: This method used μ Bondapak C18 column with methanol as mobile phase, 1.0 ml/min as flow and 996-photodiode array detector at 208 nm. RESULTS: It can separate diosgenin and its primary impurity. The calibration curve was linear (r=0.998 5) in the range of 1.90～9.51 μg for diosgenin. The average recovery and precision (RSD) for diosgenin were 96.15%(n=5) and 1.72%(n=5) respectively. CONCLUSION: Using the method derivatization is not needed in determining sample and the result of separation is good. The analysis is accurate, and satisfactory.关键词
穿龙薯蓣总皂甙/薯蓣皂甙元/反相高效液相色谱/二极管阵列检测器分类
医药卫生引用本文复制引用
都述虎,王晓华,夏重道,刘文英..RP-HPLC法测定穿龙薯蓣总皂甙中薯蓣皂甙元的含量[J].中国药科大学学报,2001,32(1):37-40,4.
