中德临床肿瘤学杂志（英文版）2004，Vol.3Issue(1)：37-41,5.
p21WAF1/CIPI基因DNA序列分析及其与骨肉瘤表型的关系
p21WAF1/CIP1 Gene DNA Sequence Change and Their Relationship with the Phenotype of Human Osteosarcoma
张春林 1廖威明 1李佛保 1曾炳芳 1曾益新1
作者信息
- 1. 上海交通大学附属第六人民医院骨科,200233
- 折叠
摘要
Abstract
Objective: To investigate the p21WAF1/CIP1 gene DNA sequence change and their relationship with the phenotype of human osteosarcoma. Methods: p21WAF1/CIP1 gene DNA of 36 osteosarcoma spec-imens was examined by using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method. The PCR products were sequenced directly. Results: In p21WAF1/CIP1 gene exon3 of 36 cases of human osteosarcoma, the change of C→T in the p21WAF1/CIP1 gene CDNA sequence of position609th occurred in 17 cases with the incidence being 44.4%. In 10 normal blood samples, DNA sequence analysis showed the change of C→T in the p21WAF1/CIP1 gene CDNA sequence of position 609th occurred in8 cases with the in cidence being 80%. Conclusion: The novel location of p21WAF1/CIP1 gene polymorphism of osteosarcoma, but not mutation was defined, and this location might provide the meaningful reference for the further research of p21WAF1/CIP1 gene.关键词
p21WAF1/CIP1基因/骨肉瘤/PCR-SSCP/DNA测序Key words
p21WAF1/CIP1 gene/osteosarcoma/PCR-SSCP/DNA sequencing分类
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张春林,廖威明,李佛保,曾炳芳,曾益新..p21WAF1/CIPI基因DNA序列分析及其与骨肉瘤表型的关系[J].中德临床肿瘤学杂志（英文版）,2004,3(1):37-41,5.
