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Development of Transgenic Restorer of Cytoplasmic Male Sterility in Upland Cotton

WANG Xue-de LI Yue-you

中国农业科学(英文版)2002,Vol.1Issue(4):375-380,6.
中国农业科学(英文版)2002,Vol.1Issue(4):375-380,6.

Development of Transgenic Restorer of Cytoplasmic Male Sterility in Upland Cotton

Development of Transgenic Restorer of Cytoplasmic Male Sterility in Upland Cotton

WANG Xue-de 1LI Yue-you1

作者信息

  • 1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, P.R. China
  • 折叠

摘要

Abstract

A glutathione S-transferase gene (gst) has been introduced into restorers of cytoplasmic male sterility in upland cotton ( Gossypium hirsutum L. ) using Agrobacterium-mediated transformation. A transviable pollens of hybrid (sterile line×restorer) F1. The hybrid gave 3.6 more bolls per plant, 10.1% less aborted seeds and 10.6% more lint yield when ‘Zheda strong restorer’ was used as male parent than when ‘DES-HAF277’ was. Southern and Northern bloting analysis showed that the foreign gst gene was detectable and highly expressed in ‘Zheda strong restorer’.

关键词

Upland cotton/Cytoplasmic male sterility/Transgenic restorer

Key words

Upland cotton/Cytoplasmic male sterility/Transgenic restorer

引用本文复制引用

WANG Xue-de,LI Yue-you..Development of Transgenic Restorer of Cytoplasmic Male Sterility in Upland Cotton[J].中国农业科学(英文版),2002,1(4):375-380,6.

基金项目

This research was supported by grants from the National Program of Plant Gene Transfer (J00-B002-10 ), the National High Technology Research and Development Program of China (863 Program,2001AA212081), and the Research Programs of the Ministry of Educatio (J00-B002-10 )

中国农业科学(英文版)

2095-3119

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