首页|期刊导航|中国农业科学(英文版)|Development of Transgenic Restorer of Cytoplasmic Male Sterility in Upland Cotton
中国农业科学(英文版)2002,Vol.1Issue(4):375-380,6.
Development of Transgenic Restorer of Cytoplasmic Male Sterility in Upland Cotton
Development of Transgenic Restorer of Cytoplasmic Male Sterility in Upland Cotton
摘要
Abstract
A glutathione S-transferase gene (gst) has been introduced into restorers of cytoplasmic male sterility in upland cotton ( Gossypium hirsutum L. ) using Agrobacterium-mediated transformation. A transviable pollens of hybrid (sterile line×restorer) F1. The hybrid gave 3.6 more bolls per plant, 10.1% less aborted seeds and 10.6% more lint yield when ‘Zheda strong restorer’ was used as male parent than when ‘DES-HAF277’ was. Southern and Northern bloting analysis showed that the foreign gst gene was detectable and highly expressed in ‘Zheda strong restorer’.关键词
Upland cotton/Cytoplasmic male sterility/Transgenic restorerKey words
Upland cotton/Cytoplasmic male sterility/Transgenic restorer引用本文复制引用
WANG Xue-de,LI Yue-you..Development of Transgenic Restorer of Cytoplasmic Male Sterility in Upland Cotton[J].中国农业科学(英文版),2002,1(4):375-380,6.基金项目
This research was supported by grants from the National Program of Plant Gene Transfer (J00-B002-10 ), the National High Technology Research and Development Program of China (863 Program,2001AA212081), and the Research Programs of the Ministry of Educatio (J00-B002-10 )