北京大学学报（医学版）2009，Vol.41Issue(3)：376-379,4.
孤啡肽基因敲除小鼠电针镇痛作用增强
Electroacupuncture analgesia is enhanced in transgenic nociceptin/orphanin FQ knock-out mice
摘要
Abstract
Objective: The modulatory effects of nociceptin/orphanin FQ ( OFQ) on acupuncture ( EA, a odern version of acupuncture)-induced analgesia are still controversial. Transgenic OFQ knock-out mice rovide us a useful tool to investigate the role of endogenous OFQ in EA analgesia. The present tudy aims to investigate the role of OFQ in the EA-induced analgesia with OFQ knock-out mice. ethods; Acupoints were selected as "Zusanli" (ST36) and "Sanyinjiao" (SP6) , EA parameters were as ollows; constant current output, rectangular (square) wave pulses, increased intensities of 0. 5, 0. 7,0. 9 mA with stepwise fashion, 10 min for each intensity, frequency of 100 Hz (with 0. 2 ms as ulse width) or 2 Hz ( with 0. 6 ms as pulse width). Tail flick latency (TFL) evoked by radiant heat was used to evaluate the change of pain threshold before, during and after EA application. The nock-out mice were divided randomly into 3 groups; needling control, EA at 100 Hz and 2 Hz. ild-type mice of litter-mates of were used as Control. Results: It was found that OFQ knock-out mice had a longer basal ther-mal threshold; EA had enhanced analgesic effect in the knock-out mice than in wild-type Control mice. Conclusion: Endogenous OFQ might be algesic in basal condition and antagonize EA analgesia.关键词
肽类/电针/针刺镇痛/痛阈/小鼠,基因敲除Key words
Peptides/Electroacupuncture/Acupuncture analgesia/Pain threshold/Mice, knock out分类
医药卫生引用本文复制引用
万有,韩济生,John E. Pintar..孤啡肽基因敲除小鼠电针镇痛作用增强[J].北京大学学报（医学版）,2009,41(3):376-379,4.基金项目
Supported by Forgorty Fellowship from NIDA/INVEST, NIH, USA （）
by grant of National Basic Research Program of China (2007CB512501) and the"111" Project of the Ministry of Education of China （2007CB512501）
