中德临床肿瘤学杂志(英文版)2006，Vol.5Issue(5)：379-382,4.
体外Fas介导细胞凋亡的细胞周期特异性的检测方法
The approaches in detecting cell cycle specificity of Fas-mediated apoptosis in leukemia cell lines and activated PBLs in vitro
摘要
Abstract
Objective: To establish a system in detecting the cell cycle specificity induced by recombinant human Fas ligand in vitro,so as to provide a reliable platform for further exploring the mechanism of cell cycle control and regulation in Fas-mediated apoptosis.Methods: The target cells-leukaemia cell lines and activated peripheral blood lymphocytes stimulated by phytohemagglutinin were incubated with recombinant human Fas ligand for 6 to 36 h,apoptosis was detected by sub-G1,common annexin-V/PI and modified annexin V and propidium iodide (API) methods and analysed by flow cytometry.Results: The modified API method demonstrated that Fas-mediated apoptosis was cell cycle specific and initiated at G1 phase.The common annexinV/PI method showed the most appropriate condition for the detection of typical cell cycle-specific apoptosis.The sub-G1 method could only illuminate late apoptosis and DNA histogram.Conclusion: Fas-mediated apoptosis was cell cycle-specific and initiated at G1 phase.Based on the modified APl and common AnnexinV/PI methods,the establishment of stable and typical cell cycle-specific model in Fas-mediated apoptosis in vitro was feasible.关键词
Fas-mediated apoptosis/cell cycle/flow cytometry/leukaemia cell/peripheral blood lymphocyteKey words
Fas-mediated apoptosis/cell cycle/flow cytometry/leukaemia cell/peripheral blood lymphocyte分类
医药卫生引用本文复制引用
何小军,胡静,李小兰..体外Fas介导细胞凋亡的细胞周期特异性的检测方法[J].中德临床肿瘤学杂志(英文版),2006,5(5):379-382,4.基金项目
Supported by the Major State Basic Research Development Program of China (973 program) (No.2004CB518705,2002CB513100-2) and Clinical Key Subject Foundation from Ministry of Health of China "Cell Cycle Diag nosis and Analysis in Clinical Tumor (Ⅲ)". （973 program）
