中国药理学与毒理学杂志2003，Vol.17Issue(5)：380-383,4.
人的基因工程氨酰基脯氨酸二肽酶的多效酶活性
Pleiotropic enzyme activities of genetically engineered human prolidase
摘要
Abstract
AIM To investigate whether the human pro- lidase possesses the G-type organophosphate hydrolyzing enzyme(Gase) activity besides its ability to catalyze the hydrolysis of the dipeptides bearing a proline residue at the C-terminus. METHODS Genetic engineering techniques were used in the cloning and expression of the recombinant human prolidase. Prolidase and Gase activities were assayed in the conventional ways. RESULTS The recombinant human prolidase expressed in COS-7 cells catalyzed the hydrolysis of organophosphorous compound soman as well as the hydrolysis of dipeptide Gly-Pro. Both activities were two-folds higher than that in the non-transformed COS-7 counterpart. Comparison between the two activities in COS-7 cells transfected with the recombinant vector containing the prolidase gene and the control cells showed parallel elevation with a constant ratio. CONCLUSION It is infered that the Gase and the prolidase are of the same enzyme, or at least belong to isozyme.关键词
水解酶类,有机磷化合物/氨酰基脯氨酸二肽酶/肝/二肽类/梭曼/人Key words
hydrolases, organophosphorous compounds/prolidase/liver/dipeptides/soman/human分类
生物科学引用本文复制引用
王青定,池木根,李前,孙曼霁..人的基因工程氨酰基脯氨酸二肽酶的多效酶活性[J].中国药理学与毒理学杂志,2003,17(5):380-383,4.基金项目
"九五"全军医药卫生科研基金资助项目(96-z-016) （96-z-016）
