吉林大学学报（医学版）2003，Vol.29Issue(4)：381-384,4.
Evaluation of proliferative activities in Wilms'tumor
Evaluation of proliferative activities in Wilms'tumor
摘要
Abstract
Objective: To assess the potential significance of proliferating cell nuclear antigen (PCNA) labelling indexes (PCNA-LI) and AgNORs number in evaluation of proliferative activity of Wilms′ tumor. Methods: A silver staining for AgNORs and an immunohistochemical method PCNA staining were performed on the biopsy specimens taken from 34 children with Wilms′ tumor. Results: No significant differences were observed between PCNA-LI and the pathological types and clinical stages, whereas fraction of S-phase and PI and the number of AgNORs were significantly higher in patients with PCNA-LI≥25% than that in patients with PCNA-LI<25%. The number of AgNORs/cell correlated well with both pathological types and clinical stages. The combination of PCNA-LI and AgNORs can accurately reflect the proliferative activity of cancer cells in Wilms′ tumor. Conclusion: The current pathological types and clinical stages may reflect the aggressive activity in Wilms′ tumor, but insufficient. The simultaneous determination of PCNA-LI and AgNORs count could be used as the essential complementarity of conventional pathological types and stages for accurate evaluation of biologic behaviour of Wilms′ tumor.关键词
肾母细胞瘤/增殖细胞核抗原/核仁组成区嗜银蛋白,流式细胞术Key words
Nephroblastoma/Proliferating cell nuclear antigen/AgNORs/Flow cytometry分类
医药卫生引用本文复制引用
WANG Lin,ZHOU Xiao-Yan,CUI Ya-Nan,ZHANG Gui-Zhen,CHEN Yuan-Yao..Evaluation of proliferative activities in Wilms'tumor[J].吉林大学学报（医学版）,2003,29(4):381-384,4.基金项目
This work was supported in part by Jilin provincial applying basic research projects(926031) （926031）
