分析化学2001，Vol.29Issue(4)：383-386,4.
活化高分子膜免疫电极检测乳腺癌抗原
Determination of Cancer Antigen 15-3 Using an Activated Polymer Membrane Immunoelectrode
摘要
Abstract
A potentiometric immunoelectrode with non-labeled anticancer antigen CA 15-3 antibody used for determinating CA15-3 was reported. firstly, the anti-CA15-3 antibody was immobilized on the surface of polyvinyl chloride-bovine serum albumin membrane, which was activated previously. Secondly, CA15-3 was added on the surface of the antibody membrane to form the antigen-antibody complex. Lastly, the complex membrane was used to assemble an immunoelectrode with an Ag-AgCl inner electrode. The potential of the complex membrane was measured with a pH meter. The response was linear over the range of 15～240 U／mL with a correlation coefficient of 0.9998. Other common antigens in human serum did not interfere with the determination of CA15-3. The mechanism of potential response was explored.关键词
乳腺癌抗原/电位法/免疫传感器分类
化学化工引用本文复制引用
彭图治,祝方猛,程琼..活化高分子膜免疫电极检测乳腺癌抗原[J].分析化学,2001,29(4):383-386,4.基金项目
本文系中国科学院长春应用化学研究所电分析化学开放研究实验室 （）
